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Growth Factor-Primed WJ-MSC Secretome Enhances Fibroblast Expansion In Vitro.

Katia Jarquín-Yáñez1, Diana Michel Aguilar-Sandoval1, Gabriela Piñón-Zárate1

  • 1Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, Mexico.

Biomedicines
|December 30, 2025
PubMed
Summary
This summary is machine-generated.

Optimized mesenchymal stem cell secretome promotes wound healing. A concentration of 1.25 mg/mL of stimulated Wharton

Keywords:
epidermal growth factor (EGF)fibroblast growth factor (FGF)secretomewharton’s jelly cells (WJ-MSC)

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Mesenchymal stem cells (MSC) secretome is explored for wound healing in regenerative medicine.
  • Wharton's jelly MSC (WJ-MSC) offer a viable source for secretome production.
  • Stimulation with FGF and EGF optimizes WJ-MSC secretome for proliferation and anti-senescence properties.

Purpose of the Study:

  • To evaluate the efficacy of WJ-MSC secretome, stimulated with growth factors, on human fibroblast proliferation and wound closure in vitro.
  • To determine the optimal concentration of stimulated WJ-MSC secretome for therapeutic applications in wound healing.

Main Methods:

  • Collected and characterized WJ-MSC secretome, both stimulated (W FGF/EGF) and unstimulated (WO FGF/EGF).
  • Assessed WJ-MSC immunophenotype and quantified growth factor content via flow cytometry and LEGENDplex.
  • Cultured human fibroblasts and treated with varying concentrations (1.25 mg/mL and 6.25 mg/mL) of secretome to assess proliferation, metabolism, and wound closure.

Main Results:

  • WJ-MSC immunophenotype remained stable post-stimulation; growth factor production significantly increased.
  • The optimal concentration for enhanced fibroblast proliferation and in vitro wound closure was 1.25 mg/mL of stimulated WJ-MSC secretome.
  • Stimulated secretome demonstrated superior efficacy compared to unstimulated secretome at equivalent concentrations.

Conclusions:

  • FGF and EGF stimulation effectively enhances growth factor secretion in WJ-MSC secretome.
  • A concentration of 1.25 mg/mL of stimulated WJ-MSC secretome is optimal for promoting fibroblast proliferation and wound closure.
  • Optimized WJ-MSC secretome presents a promising acellular therapeutic strategy for regenerative medicine and wound healing applications.