Walnut Protein Peptide Nanoparticles with Protective Mineralization: Resveratrol Encapsulation, Intestinal-Targeted...

Area of Science:

• Food Science and Technology
• Biomaterials Engineering
• Nutraceuticals

Background:

• Resveratrol (RES) exhibits poor bioavailability and gastrointestinal stability, hindering its health benefits.
• Developing effective delivery systems is crucial for maximizing RES efficacy in functional foods.

Purpose of the Study:

• To develop biomimetic mineralized nanoparticles for enhanced intestinal delivery of resveratrol.
• To investigate the impact of mineralization on nanoparticle stability, resveratrol release, and antioxidant activity.

Main Methods:

• Walnut protein peptides (WPP) were optimized for RES encapsulation.
• Mineralization of WPP-RES nanoparticles with calcium phosphate (CaP) was performed.
• In vitro gastrointestinal digestion and antioxidant assays (DPPH, ABTS) were conducted.

Main Results:

• Mineralized WPP-RES@CaP nanoparticles achieved 95.86% encapsulation efficiency, significantly higher than non-mineralized ones (73.69%).
• The CaP shell protected RES and WPP during gastric digestion, enabling sustained intestinal release (77.57% vs. 49.73%).
• WPP-RES@CaP exhibited enhanced antioxidant activity due to synergistic effects of released RES, peptides, and amino acids.

Conclusions:

• Biomimetic mineralized nanoparticles offer a promising strategy for improving the delivery and efficacy of hydrophobic compounds like resveratrol.
• The WPP-RES@CaP system enhances resveratrol's stability and targeted intestinal release, leading to improved antioxidant properties.
• This approach holds potential for developing advanced functional foods with enhanced health benefits.