Comprehensive Updated Genome-Wide Identification and Expression Patterns of the TaGeBP Gene Family in Wheat

Area of Science:

• Plant Molecular Biology
• Genomics
• Plant Physiology

Background:

• The GLABROUS1 Enhancer Binding Protein (GeBP) family are plant-specific transcription factors vital for development and stress responses.
• Previous studies identified 11 GeBP genes in wheat, but a comprehensive analysis was lacking.

Purpose of the Study:

• To conduct a comprehensive genome-wide identification and systematic analysis of the TaGeBP gene family in common wheat (Triticum aestivum L.).
• To investigate the evolutionary, expression, and functional roles of TaGeBP genes, particularly in response to abiotic stress.

Main Methods:

• Genome-wide identification of TaGeBP genes using updated wheat genome assemblies.
• Phylogenetic analysis, exon-intron structure, protein motif analysis, and promoter cis-acting element analysis.
• Tissue-specific expression profiling, quantitative real-time PCR (qRT-PCR) under stress conditions, and subcellular localization.

Main Results:

• Identified 37 TaGeBP genes, significantly more than previously reported, classified into four distinct phylogenetic groups.
• Promoter analysis revealed enrichment of stress and phytohormone-responsive elements, suggesting roles in plant adaptation.
• Expression profiling showed preferential root and spike expression, with diverse responses to salt and drought stress; nuclear localization confirmed their transcription factor function.

Conclusions:

• The TaGeBP family expansion is attributed to allopolyploidization and segmental duplication, with evolution shaped by purifying selection.
• TaGeBP genes are implicated in mediating plant adaptive responses to abiotic stresses through phytohormone signaling.
• This study provides a foundation for understanding the functional significance of TaGeBP genes in wheat's abiotic stress tolerance.