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Optimized Laboratory Maintenance and Functional Testing of Chara braunii.

Katarina Kurtović1, Jan Petrášek1,2, Stanislav Vosolsobě1

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|December 30, 2025
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Summary

We developed cost-effective protocols for cultivating and germinating Chara braunii (C. braunii) NIES-1604. These methods enable continuous growth and efficient oospore germination, advancing its use as a model organism.

Keywords:
Chara brauniiRaspberry PIcultivationgerminationin vivo stainingpropagationstreptophyte algae

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Evolutionary Biology

Background:

  • Chara braunii is an emerging model organism for plant evolution and development studies.
  • Previous research was limited by challenges like epiphyte overgrowth and non-continuous growth.
  • Established Chara species have been vital for electrophysiology, cytoplasmic streaming, and cell biology research.

Purpose of the Study:

  • To present optimized protocols for C. braunii NIES-1604 cultivation, germination, and in vivo staining.
  • To overcome limitations hindering its use as a model organism.
  • To facilitate laboratory cultivation and advance C. braunii research.

Main Methods:

  • Construction of a custom cultivation chamber simulating natural conditions for year-round growth.
  • Determination of optimal stratification periods for C. braunii NIES-1604 oospore germination.
  • Development of fluorescence-based in vivo staining protocols for cellular structures.

Main Results:

  • Achieved a 26% germination rate for C. braunii NIES-1604 oospores.
  • Enabled precise simulation of natural conditions for continuous C. braunii cultivation.
  • Established routine in vivo staining methods for plasma membrane, cell wall, and nuclei.

Conclusions:

  • The presented affordable methods provide a valuable resource for establishing Chara cultures.
  • These protocols overcome limitations in C. braunii cultivation and germination.
  • The study advances the utility of C. braunii NIES-1604 as a model organism in plant science.