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Updated: Jan 7, 2026
Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
Published on: August 22, 2007
Protocol for fabrication and utilization of the device for axon-cancer cell interaction testing
Ines Velazquez-Quesada1, Kara Allison1, Vahid Alizadeh1
1Bioengineering Department, Temple University, Philadelphia, PA 19122, USA.
None:
The device for axon and cancer cell interaction testing (DACIT) mimics neuron-cancer interactions by compartmentalizing neuron soma and axons. Here, we present a protocol to design, fabricate, load, and image the DACIT. We describe steps for DACIT master generation, smooth-on mold replication, and DACIT fabrication. We then detail procedures for cell loading in 2D and 3D settings. This protocol also includes custom 3D-printed imaging holders. For complete details on the use and execution of this protocol, please refer to Velazquez-Quesada et al.1.
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