Related Experiment Video
Updated: Jan 7, 2026
Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice
Published on: February 2, 2019
Acute effects of high-intensity interval training on adiponectin isoforms in inactive young adults: a
Daniela Alvarado-Carrasco1, Antonio González-Maraboli1, María Reyes-Montalva1
1Escuela de Kinesiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile.
Background:
Physical inactivity is a key risk factor for metabolic disorders. High-intensity interval training (HIIT) has been recognized for its metabolic benefits, but its acute effects on adiponectin isoforms remain unclear. This study examines the impact of a single HIIT session on circulating adiponectin (high-molecular weight; HMW, medium-molecular-weight; MMW, and low-molecular weight; LMW) isoforms and metabolic outcomes in insufficiently active young adults.
Methods:
A quasi-experimental study was conducted with 21 physically inactive adults (11 women, 10 men, mean age 22.7 ± 1.5 years). Participants performed a HIIT session consisting of six bouts at 80% heart rate reserve (HRR). Pre- and post-exercise blood samples were ana-lyzed for adiponectin isoforms via Western blotting, and metabolic markers were assessed. Statistical analyses included Wilcoxon tests.
Results:
MMW adiponectin significantly increased (~ 19%; effect size [95%CI]: 0.49 [-0.02-1.00] p < 0.05), while LMW and HMW adiponectin remained unchanged. The HMW/MMW ratio decreased (-16%; effect size [95%CI]: -0.49 [-0.99-0.00] p < 0.05). Additionally, non-HDL cholesterol (effect size [95%CI]: 0.464 [-0.03-0.958]) and lactate levels (effect size [95%CI]: 1.164 [0.568-1.759]) increased post-exercise (both p < 0.05).
Conclusions:
A single HIIT session acutely increases MMW adiponectin in insufficiently active young adults. Further research is needed to determine the long-term metabolic implications of repeated HIIT sessions on adiponectin isoform dynamics.
Trial Registration:
retrospectively registered in ClinicalTrials.gov (NCT07146867) on August 21st, 2025.
