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Discovering Anticancer Effects of Phytochemicals on MicroRNA in the Context of Data Mining

Yumi Sakai1,2, Kurataka Otsuka1,2,3,4

  • 1Laboratory of Biomolecular Signaling, Tokyo NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.

Nutrients
|December 31, 2025
PubMed

Insights

Artificial intelligence identified novel phytochemicals, including fisetin, glabridin, and silibinin, that regulate microRNAs (miRNAs) in colon cancer cells. These compounds show potential for diet-based disease prevention strategies.

Area of Science:

  • Nutritional Science
  • Molecular Biology
  • Oncology

Background:

  • MicroRNAs (miRNAs) are implicated in various human diseases, including cancer, and their expression profiles are linked to disease prevention.
  • Understanding how micro- and macronutrients influence miRNA expression is crucial for health maintenance.
  • Identifying specific phytochemicals that modulate miRNA expression is key to discovering new dietary functions.

Purpose of the Study:

  • To identify novel phytochemicals that regulate microRNAs (miRNAs) in colon cancer using artificial intelligence.
  • To analyze miRNA expression changes in colon cancer cells treated with new phytochemical candidates.
  • To explore the potential of these phytochemicals in diet-based health promotion and disease prevention.

Main Methods:

  • Utilized artificial intelligence to screen for phytochemicals regulating miRNAs in colon cancer.
  • Conducted next-generation sequencing to analyze miRNA expression in colon cancer cell lines treated with phytochemical candidates.
  • Performed pathway analyses to understand the molecular mechanisms affected by the identified phytochemicals.

Main Results:

  • Identified three phytochemicals—fisetin, glabridin, and silibinin—that suppressed colon cancer cell proliferation and altered cancer-related miRNA expression.
  • Observed both common and compound-specific miRNA expression signatures in response to each phytochemical.
  • Found that these phytochemicals impact key tumor development pathways, including p53 signaling, apoptosis, and cellular senescence.

Conclusions:

  • Artificial intelligence is effective in discovering new phytochemicals that modulate tumor-related miRNAs.
  • Fisetin, glabridin, and silibinin demonstrate potential as dietary agents influencing miRNA expression in colon cancer.
  • Further research into miRNA mechanisms can enhance understanding of dietary nutrient functions for health promotion and disease prevention.
Keywords:
colorectal cancerdietfisetinglabridinmicroRNAphytochemicalsilibinin

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