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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
Published on: February 18, 2014
The Transition State of PBLG Studied by Deuterium NMR
Fabian M Hoffmann1, Burkhard Luy1
1Institute for Biological Interfaces 4 (IBG-4), Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.
Abstract:
The liquid crystal (LC) poly-γ-benzyl-L-glutamate (PBLG) is known to possess a narrow biphasic range at the phase transition from an isotropic liquid to an anisotropic liquid crystal. We have characterized the biphasic region via deuterium nuclear magnetic resonance (NMR) of the deuterated solvent CDCl3, with which isotropic and anisotropic populations can unambiguously be identified and quantified due to the quadrupolar coupling induced by partial alignment. In addition to a dilution series, we measured the kinetics of the alignment inside the magnet for each dilution step and were able to follow the kinetic buildup of partial alignment. Beginning with the dynamic line broadening indicative of slow fluctuations, to microheterogeneous patches of isotropic and anisotropic islands, with increasing island size being consistent with sharpened spectra, ending in fully separated isotropic and anisotropic phases on top of each other after two weeks. In addition, we studied the influence of the two example guest molecules borneol and camphor-which essentially differ in their capability to act as hydrogen bond donors or acceptors-on the biphasic region of PBLG.
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