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  3. Lowcost-hsqc Variants For Fast-pulsing High Ω1-resolved 2d Experiments.
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  3. Lowcost-hsqc Variants For Fast-pulsing High Ω1-resolved 2d Experiments.

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LowCOST-HSQC Variants for Fast-Pulsing High ω1-Resolved 2D Experiments.

David Schulze-Sünninghausen1, Johanna Becker2, Martin R M Koos3

  • 1Bruker BioSpin GmbH & Co. KG, Ettlingen, Germany.

Magnetic Resonance in Chemistry : MRC
|April 16, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

The LowCOST-HSQC method enhances sensitivity for low-abundance nuclei like Carbon-13 and Nitrogen-15. This technique enables faster 2D Nuclear Magnetic Resonance experiments by retaining proton polarization.

Keywords:
ASAPHSQCLowCOSTTIG‐BIRDZIPfast‐pulsing 2D NMR

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Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Biophysical Chemistry

Background:

  • Sensitivity enhancement is crucial for analyzing low-abundance nuclei (e.g., Carbon-13, Nitrogen-15) in biomolecules.
  • Standard HSQC experiments can be time-consuming due to limited sensitivity.

Purpose of the Study:

  • To introduce and compare INEPT-type transfer elements for the Low-field Optimized Constant-time Sensitivity Transfer-Heteronuclear Single Quantum Coherence (LowCOST-HSQC) experiment.
  • To evaluate different variants of the LowCOST-HSQC pulse sequence.

Main Methods:

  • Detailed theoretical description of three INEPT-type transfer elements: original LowCOST, ZIP, and TIG-BIRD.
  • Evaluation of various LowCOST-HSQC pulse sequence configurations.
  • Utilizing fast polarization distribution via isotropic mixing for rapid 2D experiment acquisition.

Main Results:

  • Demonstrated that LowCOST-HSQC retains unused proton polarization for improved sensitivity.
  • Showcased the capability for fast-pulsing 2D experiments.
  • Provided a comparative analysis of different INEPT-type transfer elements within the LowCOST framework.

Conclusions:

  • LowCOST-HSQC is an effective sensitivity-enhanced technique for correlating with low natural abundance nuclei.
  • The choice of INEPT-type transfer element impacts the performance of LowCOST-HSQC experiments.
  • This method facilitates faster acquisition of 2D NMR data, particularly for challenging samples.