Development of a Method for the Detection, Quantification, and Validation of 4-Oxo-2-Nonenal in Cooked Meat

This research mainly focused on developing and validating the ultraperformance liquid chromatography photodiode array (UPLC-PDA) method to analyze 4-oxo-2-nonenal (4-ONE) in fully cooked meat products. The UPLC-PDA method developed has advantages such as sensitivity, excellent resolution, and fast separation through chromatography. To ensure its reliability and accuracy, the method underwent validation processes for linearity, precision, accuracy, robustness, and limit of detection (LOD) and quantification (LOQ). The results demonstrated a linear relationship between concentration and response within the 0.0032-10 ng/mL range with a correlation coefficient of R ² ≥ 0.9993. The method also exhibited precision with relative standard deviations (RSDs) below 2% for both intraday and interday analyses. Moreover, recovery studies confirmed the method's accuracy, with percent recoveries ranging from 97.16% to 105.9%. Furthermore, the results for LOD and LOQ were 0.03 and 0.091 ng/mL, respectively. Lastly, it was concluded that the developed method remains reliable under certain conditions by varying parameters such as the flow rate, mobile phase composition, and detection wavelength in robustness evaluations. This developed UPLC-PDA technique offers a reliable and effective means of identifying and measuring 4-ONE in cooked meat. It plays a role in ensuring food safety and addressing health issues associated with its consumption.