Search research articles

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A minimal mechanically consistent model of smoothly dividing disk-shaped cells.

NPJ systems biology and applications·2026

Same author

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Signal transduction and targeted therapy·2026

Same author

Left atrial appendage occluder thrombosis caused embolic strokes: a case report.

European heart journal. Case reports·2026

Same author

Stem-Cell-Derived Biologic Ventricular Assist Tissue in Heart Failure.

The New England journal of medicine·2026

Same author

Quantifying 3D live-cell membrane dynamics using dynamic metal-induced energy transfer spectroscopy (dynaMIET).

Science advances·2026

Same author

NanoPlex: A Universal Strategy to Multiplex Immunostainings for Fluorescence Microscopy Using Secondary Nanobodies.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Formation of Bimetallic Nanoparticles via Exsolution Using a Reducible Metal Oxide Capping Layer.

ACS nano·2026

Same journal

Cold-Driven Thermoelectric Patch for Postoperative Tumor Control.

ACS nano·2026

Same journal

Chemically Fueled Interfacial Supramolecular Polymerization.

ACS nano·2026

Same journal

Tactile Neuromorphic Ion-Gated Vertical Transistor Displays Enabling Dual-Output Reservoir Computing.

ACS nano·2026

Same journal

In Situ Oxygen Shuttling within a Bilayer Electrified Membrane Enables Aeration-Free Electro-Fenton Water Purification.

ACS nano·2026

Same journal

Single Atoms as Growth Directors: From Graphene Edges to Atomically Precise Interfaces in 2D Materials.

ACS nano·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jan 7, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy.

Samrat Basak^1,2, Kim-Chi Vu^3,4,5, Nikolaos Mougios^6,7

¹III. Institute of Physics - Biophysics, Georg August University, 37077 Göttingen, Germany.

|January 1, 2026

Summary

This summary is machine-generated.

We developed a microfluidics system for DNA-PAINT super-resolution microscopy, improving multiplexed imaging efficiency and reproducibility. This automated platform enables nanoscale imaging in complex biological samples like cells.

Keywords:

DNA-PAINT SMLM automation cardiomyocytes microfluidics multiplexed imaging single-molecule localization microscopy super-resolution microscopy

More Related Videos

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Related Experiment Videos

Last Updated: Jan 7, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Area of Science:

Super-resolution microscopy
Nanoscale imaging
Biophysics

Background:

DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a single-molecule localization microscopy (SMLM) technique.
Exchange-PAINT enables multiplexing by sequential target imaging using orthogonal DNA strands.
Manual Exchange-PAINT workflows suffer from inefficiency, drift, variability, and poor reproducibility.

Purpose of the Study:

To develop an automated, microfluidics-based system for enhanced multiplexed SMLM.
To improve the efficiency, reproducibility, and material usage of Exchange-PAINT.
To enable reliable nanoscale imaging in complex biological systems.

Main Methods:

A custom compressed-air-driven microfluidics system with a stackable, modular design.
Robust and material-efficient buffer exchange with minimal dead volume.
Adaptable to various SMLM platforms (wide-field, confocal, STED, MINFLUX).

Main Results:

Demonstrated robust 5-plex Exchange-PAINT imaging in U2OS cancer cells.
Successfully performed multiplexed nanoscale imaging in fragile primary cardiomyocytes.
The system ensures reproducible buffer exchange and minimal dead volume.

Conclusions:

The microfluidics platform enables reliable super-resolution multiplexing in physiologically relevant systems.
Facilitates detailed nanoscale analysis in complex primary cells.
Offers a versatile solution for advanced SMLM applications.