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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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SPARK-seq: A high-throughput platform for aptamer discovery and kinetic profiling.

Guoyan Luo1,2, Jia Song3,4, Yongbo Fu3,5

  • 1Department of Pathology, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, P.R. China.

Science (New York, N.Y.)
|January 1, 2026
PubMed
Summary

Researchers developed SPARK-seq, a new method for discovering aptamers that bind to cell surface proteins. This high-throughput platform enables efficient identification and engineering of aptamers for diagnostics and therapeutics.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Cell surface proteins are crucial biomarkers and therapeutic targets.
  • Existing high-throughput aptamer discovery methods for in situ targeting are limited.

Purpose of the Study:

  • To introduce SPARK-seq, a novel high-throughput platform for aptamer discovery against cell surface proteins.
  • To enable simultaneous mapping of aptamers to multiple proteins and capture diverse binding interactions.

Main Methods:

  • SPARK-seq integrates single-cell RNA and aptamer sequencing with CRISPR-based surface protein perturbation.
  • The platform captures aptamer-protein interactions across a wide range of protein abundances and biophysical classes.

Main Results:

  • Simultaneously mapped 5,535 aptamers to eight surface proteins in a single experiment.
  • Achieved discrimination of closely related paralogous proteins with no cross-reactivity.
  • Obtained kinetic information for aptamer prioritization and engineered variants with improved off-rate properties.

Conclusions:

  • SPARK-seq provides a high-efficiency platform for aptamer discovery and rational variant design.
  • The method advances the development of aptamers for diagnostics and therapeutics targeting cell surface proteins.