Application of Synchrotron Radiation Circular Dichroism for mRNA Structural Analysis
Ayoub Medjmedj1, Josef Hamacek1, Frank Wien2
1Centre de Biophysique Moléculaire CNRS UPR4301, Orléans, France.
Abstract:
Circular dichroism is a useful tool to study the impact of mRNA sequence optimization on mRNA structure. mRNA can fold into several conformations according to the sequence of nucleotides. Structural characteristics of mRNA (melting temperature, unfolding trajectories) are important for their biological function. Circular dichroism is a powerful technique to characterize mRNA structure. In this chapter we describe how to analyze protein coding mRNA by Synchrotron Radiation Circular Dichroism (SRCD). The emphasis is put on mRNA synthesis and sample preparation, which are critical for mRNA analysis. We also provide a protocol for the in vivo evaluation of vaccine activity.
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