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Related Experiment Video

Updated: May 4, 2026

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
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Whole-Genome Amplification on Single Circulating Trophoblast Cell.

Imen Chakchouk1,2, Ignatia B Van den Veyver3,4

  • 1Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
PubMed
Summary

Whole-genome amplification (WGA) enables genetic analysis from single cells. This protocol details WGA for circulating trophoblast cells in cell-based noninvasive prenatal testing (cb-NIPT).

Keywords:
Maternal bloodSingle circulating trophoblastWhole-genome amplification

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Area of Science:

  • Genetics
  • Molecular Biology
  • Reproductive Medicine

Background:

  • Whole-genome amplification (WGA) is crucial for analyzing limited DNA samples, such as single cells.
  • Cell-based noninvasive prenatal testing (cb-NIPT) relies on analyzing fetal DNA from maternal blood.
  • Existing WGA methods may conflict with single trophoblast isolation protocols.

Purpose of the Study:

  • To provide a detailed protocol for WGA on single circulating trophoblast cells.
  • To optimize WGA for compatibility with cb-NIPT workflows.
  • To facilitate comprehensive genetic analysis from trace amounts of DNA.

Main Methods:

  • Isolation of single circulating trophoblast cells from maternal blood.
  • Application of a WGA technique compatible with trophoblast isolation steps (fixation, permeabilization, immunostaining).
  • SNP genotyping and next-generation sequencing of amplified DNA.

Main Results:

  • Successful amplification of the entire genome from isolated single trophoblast cells.
  • Generation of sufficient DNA for downstream genetic analysis.
  • Demonstration of a viable protocol for cb-NIPT.

Conclusions:

  • This protocol enables WGA on single circulating trophoblasts for cb-NIPT.
  • The method supports comprehensive genetic testing from trace DNA.
  • It addresses compatibility challenges between WGA and cell isolation techniques.