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Detection of Multi-analytes Using Luminex Multiplex Bead Immunoassay
Md Asrarul Islam1, Yamin Farabih1, Sunil Kumar2
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA.
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The Luminex multiplex bead immunoassay enables the detection of more than 100 analytes in a sample at a time. This method utilizes magnetic beads coated with antibodies of interest to measure multi-analytes simultaneously. Here, we describe a detailed protocol for multi-analyte detection of two proteins, fatty acid binding protein 1 (FABP1) and fibroblast growth factor 19 (FGF19), both related to fat metabolism, in cell lysates of HepG2 hepatocytes.
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