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Updated: Jan 7, 2026

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Published on: June 9, 2019
Evaluation of Metabolic Characteristics of Cancer Cells
Riddhi Mehta1, Pritpal Kaur1, Fatema Zohra Sadia1
1Department of Biological Sciences, St. John's University, Queens, NY, USA.
Abstract:
Cancer cells undergo a complex rearrangement of metabolic pathways that allows them to satisfy the needs of increased proliferation. Since many cancers are characterized by a high glycolytic rate regardless of oxygen availability, targeting glycolysis, electron transport chain (ETC), and oxidative phosphorylation (OXPHOS) have emerged as a potential therapeutic strategy. In this chapter, we describe a protocol that utilizes the Agilent Seahorse XFp Analyzer to assess mitochondrial respiration and glycolysis in ovarian cancer cells.
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