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Related Experiment Video

Updated: May 1, 2026

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Targeting Fibrosis in Arthritis Using mRNA Vaccine-Based Approach.

Qiang Xu^1,2, Xiaofei Shi^1,3, Xiaowei Zhang¹

¹Division of Arthritis and Rheumatic Diseases, Oregon Health & Science University, Portland, OR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 1, 2026

Summary

A novel Fibroblast Activation Protein (FAP) mRNA vaccine delivered via lipid nanoparticles (LNPs) effectively reduced arthritis severity in preclinical models. This FAP mRNA-LNP vaccine shows promise for treating fibrotic diseases and cancer.

Keywords:

Arthritis Cancer Fibroblast activation protein Fibrosis mRNA vaccine

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An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

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Published on: June 6, 2025

Area of Science:

Immunology
Biotechnology
Molecular Medicine

Background:

Fibroblast Activation Protein (FAP) is highly expressed in activated fibroblasts within chronic inflammation and cancer microenvironments.
FAP is a recognized therapeutic target for fibrotic pathologies.
Activated fibroblasts play a critical role in disease progression.

Purpose of the Study:

To develop and evaluate a Fibroblast Activation Protein (FAP) mRNA vaccine encapsulated in lipid nanoparticles (FAP mRNA-LNP).
To assess the efficacy of the FAP mRNA-LNP vaccine in attenuating the severity of experimental arthritis.
To explore the potential broader applications of this vaccine technology.

Main Methods:

Development of a FAP mRNA vaccine formulated within lipid nanoparticles (LNPs).
Administration of the FAP mRNA-LNP vaccine as a potential immunotherapeutic agent.
Evaluation of vaccine efficacy in a preclinical model of experimental arthritis.

Main Results:

The FAP mRNA-LNP vaccine successfully attenuated the severity of experimental arthritis.
Demonstrated the feasibility of using mRNA-LNP technology for targeting FAP.
Indicated a potential therapeutic effect beyond arthritis.

Conclusions:

FAP mRNA-LNP vaccine is a promising strategy for managing fibrotic conditions like arthritis.
This approach holds potential for treating cancer and other FAP-associated fibrotic diseases.
Highlights the therapeutic versatility of mRNA-LNP vaccines targeting specific proteins.