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Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing
Olivia Hu1, Alessandro Provvido1, Yan Zhu2
1Department of Biological Sciences, St. John's University, Queens, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
Summary
This study details a CRISPR/Cas9 method to create knockout cell lines for interleukin-17 receptor B (IL17RB), a gene overexpressed in cancers. This efficient genome editing approach can be adapted for other gene targets.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Interleukin-17 receptor B (IL17RB) is a cytokine receptor implicated in various cancers due to its overexpression.
- CRISPR/Cas9 technology offers a precise and cost-effective method for genetic modification.
Purpose of the Study:
- To present a detailed methodological approach for generating IL17RB knockout cell lines using CRISPR/Cas9-mediated genomic deletion.
- To establish a reliable protocol for creating specific gene knockouts in mammalian cell lines.
Main Methods:
- CRISPR design and cloning for targeting the IL17RB gene.
- Delivery of CRISPR constructs into target cells.
- Verification of IL17RB gene deletion via PCR and deletion screening.
Main Results:
- Successful establishment of IL17RB knockout cell lines.
- Demonstration of an efficient CRISPR/Cas9-based genomic deletion strategy.
- Validation of the methodology for generating specific gene knockouts.
Conclusions:
- The presented method provides an effective means to generate IL17RB knockout cell lines.
- This approach is adaptable for creating knockout cell lines for other genes of interest.
- CRISPR/Cas9 technology facilitates efficient genetic modification for research applications.
