Method for Determining the Affinity of the T Cell Receptor for the Peptide-Human Leukocyte Antigen Complex
Lawton D Murdolo1,2, Stephanie Gras1,2,3, Demetra S M Chatzileontiadou4,5,6
1Infection and Immunity Program, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Bundoora, VIC, Australia.
Abstract:
T cells are key players of the cellular immune system, able to detect and fight pathogens' invasion. T cells recognize pathogen-derived peptides presented by molecules called Human Leukocyte Antigens (HLA) in humans. The interaction between the T cells and peptide-HLA (pHLA) complexes is driven by the surface T cell receptors (TCRs). This interaction is critical and the first step of T cell activation. The affinity of the TCR for the pHLA is one of the key drivers of T cell activation. In this chapter, we describe the method to determine the affinity of the TCR for the pHLA via surface plasmon resonance.
Related Concept Videos
01:22T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
01:28Diversity of Antigen Receptors
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...