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Genome sequence data of multidrug-resistant Enterococcus faecalis HMTZ24 carrying multiple virulence factors,

Talal S Salih1, Zeyad T Al-Rrassam1, Muhammad A Muhammad2

  • 1Department of Medical Physics, College of Science, University of Mosul, Iraq.

Data in Brief
|January 2, 2026
PubMed

Insights

We present the draft genome sequence of Enterococcus faecalis strain HMTZ24, isolated from a UTI patient in Iraq. This genomic data reveals antimicrobial resistance and virulence factors, aiding future research on this common pathogen.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Enterococcus faecalis is a significant cause of urinary tract infections (UTIs) globally.
  • Understanding the genetic makeup of E. faecalis strains is crucial for combating antibiotic resistance and infection spread.

Purpose of the Study:

  • To present the draft genome sequence of Enterococcus faecalis strain HMTZ24.
  • To identify antimicrobial resistance genes and virulence factors within this strain.
  • To provide a genomic resource for studying E. faecalis epidemiology and pathogenesis.

Main Methods:

  • Whole-genome sequencing using Illumina NovaSeq 6000.
  • Bioinformatic analysis including genome assembly, annotation, phylogenomic taxonomy, and MLST.
  • Identification of antimicrobial resistance genes, mobile genetic elements, and virulence factors.

Main Results:

  • The assembled genome is 2.62 Mbp with 2,510 coding sequences.
  • Strain HMTZ24 belongs to Sequence Type 28 (ST28) and is closely related to reference strains.
  • The genome contains multiple antimicrobial resistance genes (e.g., vancomycin, ciprofloxacin) and 14 virulence factors (e.g., ace, ebpA).

Conclusions:

  • The HMTZ24 genome provides valuable insights into the genetic basis of antimicrobial resistance and virulence in E. faecalis.
  • This genomic data supports comparative studies and enhances understanding of regional E. faecalis epidemiology.
  • The availability of this genome sequence facilitates further research into UTI pathogenesis and treatment strategies.
Keywords:
Antimicrobial resistanceEnterococcus faecalisGenome sequenceMobile genetic elementsPhylogenomicSequence typingVirulence

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