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N-myristoyltransferase 1-mediated Src Myristoylation Promotes Non-receptor Tyrosine Kinase Pathways in Oral Squamous

Takuma Komiyama1, Goro Sugiyama2, Hanae Kamohara1

  • 1Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

In Vivo (Athens, Greece)
|January 2, 2026
PubMed
Abstract

Insights

N-myristoyltransferase 1 (NMT1) promotes oral squamous cell carcinoma (OSCC) malignancy. NMT1 mediates Src myristoylation, a key interaction driving OSCC progression and potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • N-myristoyltransferase 1 (NMT1) is highly expressed in oral squamous cell carcinoma (OSCC).
  • Existing targeted therapies for OSCC have limitations, and the role of NMT1-Src interactions is unexplored.
  • NMT1 mediates the myristoylation of Src, a critical signaling protein.

Purpose of the Study:

  • To investigate the role of NMT1-mediated Src myristoylation in OSCC malignancy.
  • To explore NMT1 as a potential therapeutic target in OSCC.

Main Methods:

  • Assessed NMT1 expression and myristoylation using click chemistry and immunocytochemistry.
  • Utilized RNA-sequencing and western blotting to analyze gene expression and Src activity.
  • Employed co-immunoprecipitation to confirm NMT1-Src binding and siRNA to knockdown NMT1 in OSCC cell lines.

Main Results:

  • NMT1 and Src were expressed in all tested OSCC cell lines.
  • NMT1 knockdown reduced expression of genes involved in cell adhesion and angiogenesis.
  • NMT1 binds to Src during myristoylation, and NMT1 knockdown decreased Src membrane localization.

Conclusions:

  • NMT1 promotes OSCC malignancy through its mediation of Src myristoylation.
  • NMT1-Src myristoylation represents a potential therapeutic avenue for OSCC.
Keywords:
NMT1Srcintrinsic activationmyristoylationnon-receptor tyrosine kinaseoral squamous cell carcinoma

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