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Published on: August 21, 2021
Charge-Driven Bioshield Remodels Diabetic Oral Microenvironment for Accelerated Wound Healing.
Yajuan Xie1, Yunfan Zhang2, Yue Yang3
1Department of Orthodontics, National Center for Stomatology, National Clinical Research Center For Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, Beijing, China.
A novel protein bioshield effectively treats diabetic chronic wounds by neutralizing neutrophil extracellular traps (NETs) and blocking bacteria. This charge-driven strategy promotes healing in challenging oral environments.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Diabetic chronic wounds present significant healing challenges, particularly in moist oral environments.
- Neutrophil extracellular traps (NETs)-mediated inflammation, bacterial biofilm infections, and extracellular matrix defects exacerbate wound pathology.
Purpose of the Study:
- To develop a protein-based adhesive bioshield for treating diabetic chronic wounds.
- To investigate the bioshield's dual function as a bacterial barrier and NETs scavenger.
- To evaluate the bioshield's impact on focal adhesion signaling and wound healing.
Main Methods:
- Development of a protein-based adhesive bioshield utilizing high-density lysine residues for adhesion and high net charge for NETs neutralization.
- Assessment of the bioshield's physical blocking and electrostatic interaction capabilities.
- Evaluation of NETs scavenging, bacterial barrier function, and promotion of diabetic wound healing in vitro and in vivo models.
Main Results:
- The bioshield demonstrated robust adhesion in dynamic oral environments, forming a persistent antibacterial barrier.
- The bioshield effectively neutralized NETs by binding cell-free DNA (cfDNA), reducing inflammation.
- This NETs clearance reactivated focal adhesion signaling, accelerating diabetic wound healing via enhanced epithelial migration, endothelial proliferation, and angiogenesis.
Conclusions:
- A charge-driven therapeutic strategy targeting NETs-mediated inflammation offers a promising approach for diabetic wound management.
- The developed protein bioshield presents an alternative therapeutic option for chronic wounds in wet and dynamic environments.
- This study highlights the potential of biomaterials in addressing complex wound healing challenges.
