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Forensic maceration - A comparative analysis of literature and practical application
Melissa Kirbach1, Caroline Kohlt2, Wilma Ludvigsson Möller3
1Institute of Legal Medicine, Medical Faculty, University of Cologne, Melatengürtel 60/62, 50823, Cologne, Germany. melissa.kirbach@uk-koeln.de.
International Journal of Legal Medicine
|January 3, 2026
Summary
Forensic maceration methods for skeletal analysis vary widely in practice, with a lack of standardization impacting consistency. This study reviews literature and surveys global practices, highlighting the need for improved protocols.
Area of Science:
- Forensic Anthropology
- Forensic Science Methodology
- Bioarchaeology
Background:
- Soft tissue removal is crucial for accurate skeletal analysis in forensic investigations.
- Maceration, a water-soaking method, is a common technique for tissue removal but lacks standardized protocols.
- Variations in maceration practices exist across different forensic professionals and settings.
Purpose of the Study:
- To review published literature on forensic maceration techniques.
- To conduct an international survey comparing current maceration practices with literature recommendations.
- To assess the impact of maceration methods on tool mark and material trace analyses on bone.
Main Methods:
- A comprehensive literature review of 27 relevant articles on forensic maceration.
- An international survey distributed to 57 laboratories and institutes across 19 countries.
- Analysis of survey data to identify common practices, variations, and potential impacts on bone analysis.
Main Results:
- Literature indicates ongoing research and development in maceration techniques, favoring warm water and detergent-based methods.
- Survey results show general alignment with literature but reveal persistent use of aggressive methods.
- Significant lack of standardization and inclusion in standard operating procedures (SOPs) was observed globally.
Conclusions:
- Current forensic maceration practices exhibit considerable variation and a lack of standardization.
- The use of aggressive maceration methods and inconsistent SOPs can affect the reliability of skeletal analyses.
- Further research and standardization of maceration protocols are essential for consistent, efficient, and reproducible forensic casework.

