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Engineering Biomimetic Nanoparticle Performance Through Fabrication Method Selection: Turbulent Jet Mixing,
Ilana Elizarov1, Rawan Mhajne1, Ofri Vizenblit1
1Bioinspired Nano Engineering and Translational Therapeutics Lab, Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Small Methods
|January 5, 2026
Summary
Turbulent jet and microfluidic mixing create superior biomimetic nanoparticles (BNPs) for drug delivery compared to extrusion. These methods enhance stability, protein incorporation, and targeting capabilities for advanced nanotherapeutics.
Area of Science:
- Biomaterials Science
- Nanomedicine
- Drug Delivery Systems
Background:
- Nanoparticle (NP) fabrication is crucial for nanomedicine, particularly in targeted drug delivery.
- Biomimetic nanoparticles (BNPs) offer advanced drug delivery by mimicking cell membrane properties.
- Effective fabrication methods are needed to optimize BNP performance.
Purpose of the Study:
- To comparatively evaluate turbulent jet mixing, microfluidic mixing, and extrusion for producing neuron-derived BNPs (Neurosomes).
- To assess the impact of fabrication methods on BNP physicochemical and biological properties.
- To identify optimal fabrication strategies for scalable nanotherapeutic development.
Main Methods:
- Comparative analysis of three nanoparticle fabrication techniques: turbulent jet mixing, microfluidic mixing, and extrusion.
- Characterization of biomimetic nanoparticle (BNP) properties, including stability, membrane protein incorporation, and batch-to-batch reproducibility.
- Application of high-resolution LC-MS/MS proteomics to quantify membrane-associated protein profiles.
Main Results:
- Turbulent jet and microfluidic mixing yielded BNPs with superior stability, higher membrane protein incorporation, and better reproducibility than extrusion.
- Extrusion resulted in diminished BNP performance, attributed to shear-induced protein loss.
- Proteomic analysis provided quantitative insights into membrane protein profiles influenced by fabrication methods.
Conclusions:
- Fabrication techniques critically influence biomimetic nanoparticle (BNP) structure, function, and performance.
- Turbulent jet and microfluidic mixing are advantageous for producing high-quality BNPs for targeted drug delivery.
- These findings offer actionable insights for optimizing nanotherapeutic production and development.
