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Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Nonionic Surfactant as a Tool to Modify Electrospun Fiber Properties for In Vitro Fibrous Connective Tissue Models.

Katherine L Meinhold1,2, Jennifer L Robinson1,2,3,4

  • 1Department of Bioengineering, University of Washington, Seattle, Washington, USA.

Journal of Biomedical Materials Research. Part A
|January 5, 2026
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Summary
This summary is machine-generated.

Adding Span80 surfactant to poly-ε-caprolactone (PCL) electrospun scaffolds enhances meniscus tissue engineering. Low concentrations improve cell adhesion and wettability, crucial for developing effective in vitro models for meniscus regeneration.

Keywords:
electrospinningextracellular matrix modelin vitro modelnonionic surfactantprimary meniscus cells

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedic Research

Background:

  • Meniscal tears are common knee injuries leading to joint degeneration.
  • Effective in vitro models require scaffolds mimicking native meniscus structure and mechanics.
  • Poly-ε-caprolactone (PCL) scaffolds are widely used but often hydrophobic, limiting their utility.

Purpose of the Study:

  • To investigate the impact of Span80 concentration on PCL scaffold properties.
  • To evaluate how Span80 affects scaffold morphology, mechanics, and surface characteristics.
  • To assess the influence of Span80 on meniscal cell behavior and matrix production.

Main Methods:

  • Electrospinning of PCL fibers with varying Span80 concentrations (0%, 10%, 30%).
  • Fabrication of both unaligned and aligned fiber scaffolds.
  • Characterization of scaffold morphology, tensile properties, wettability, and protein adsorption.
  • Assessment of meniscal cell adhesion and extracellular matrix production.

Main Results:

  • Low Span80 (10%) increased wettability and cell adhesion/fibronectin production with minimal impact on fiber structure and mechanics.
  • High Span80 (30%) reduced fiber diameter, tensile strength, and cell viability, particularly in aligned scaffolds.
  • Span80 concentration-dependently modulated scaffold surface properties and cell interactions.

Conclusions:

  • Span80 is a useful additive for tailoring PCL scaffold properties for meniscus tissue engineering.
  • Optimized Span80 concentrations can enhance cell compatibility and create better in vitro models.
  • This approach offers an adaptable method to study cell behavior in a biomimetic environment.