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OsAE7 Interacts With ZFP36 to Mediate Antioxidant Defense in Rice.

Liping Huang1, Xiwang Xu1, Mengyao Zhang1

  • 1International Research Center for Environmental Membrane Biology, Foshan University, Foshan, China.

Physiologia Plantarum
|January 5, 2026
PubMed
Summary
This summary is machine-generated.

Rice gene OsAE7 is crucial for drought and salt stress tolerance by mediating abscisic acid (ABA) signaling. ABA induces OsAE7 expression, enhancing plant stress resistance.

Keywords:
abscisic acidasymmetric leaves1/2 enhancer 7osmotic stressprotein interactionsalinityzinc finger protein 36

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Area of Science:

  • Plant Biology
  • Molecular Plant Science
  • Plant Stress Physiology

Background:

  • Abscisic acid (ABA) is a key plant hormone regulating adaptation to abiotic stresses like drought and salinity.
  • Understanding the molecular mechanisms underlying ABA signaling is vital for improving crop resilience.

Purpose of the Study:

  • To investigate the role of rice gene OsAE7 (asymmetric leaves1/2 enhancer 7) in ABA signaling and stress response.
  • To elucidate the interaction between OsAE7 and ZFP36 (zinc finger protein 36) in rice.

Main Methods:

  • Yeast two-hybrid (Y2H), GST pull-down, and Firefly luciferase complementation imaging assay (LCI) were used to study protein interactions.
  • CRISPR/Cas9 technology was employed to generate OsAE7 knockout mutants (osae7-KO).
  • Gene expression analysis (qRT-PCR) and physiological assays were conducted under various stress conditions.

Main Results:

  • OsAE7 expression is upregulated by ABA, H2O2, osmotic stress (PEG), and NaCl, indicating its involvement in stress responses.
  • OsAE7 knockout mutants exhibited reduced stress tolerance, lower antioxidant enzyme activity, and altered physiological parameters compared to wild-type rice.
  • The interaction between OsAE7 and ZFP36 was found to influence ABA-induced OsAE7 expression.

Conclusions:

  • OsAE7 plays a significant role in rice's response to drought and salt stress, primarily through its involvement in the ABA signaling pathway.
  • OsAE7 acts as a positive regulator of stress tolerance in rice.
  • The OsAE7-ZFP36 interaction is important for mediating ABA's effect on OsAE7 expression.