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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jul 9, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

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Fluorogenic CRISPR for DNA imaging in live mammalian cells.

Wenjin Wan1, Xin Ji2, Haozhi Song2

  • 1Interdisciplinary Science Center, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.

Cell Chemical Biology
|January 6, 2026
PubMed
Summary

New fluorogenic CRISPR imaging tools offer enhanced sensitivity for tracking genomic DNA dynamics in live cells. These advanced methods overcome limitations of traditional tools, improving signal-to-noise ratios for crucial biological research.

Keywords:
CRISPRDNA imagingfluorogenic CRISPRfluorogenic probehigh contrast imaging

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • Spatiotemporal imaging of genomic DNA dynamics in live mammalian cells is crucial for understanding eukaryotic organization and processes.
  • Conventional CRISPR imaging tools often suffer from high background noise and low signal-to-noise ratios due to constitutively fluorescent proteins.

Purpose of the Study:

  • To review and summarize fluorogenic CRISPR-based strategies for live cell DNA imaging.
  • To highlight advancements in reducing background noise and enhancing sensitivity in DNA imaging.

Main Methods:

  • Review of four distinct fluorogenic CRISPR strategies utilizing different reporters: fluorogenic proteins, RNA aptamers, split fluorescent proteins, and molecular beacons.
  • Analysis of their application in monitoring subnuclear gene loci localization, dynamics, and DNA repair processes.

Main Results:

  • Fluorogenic CRISPR tools significantly reduce background noise and increase signal sensitivity by remaining non-fluorescent until target DNA binding.
  • These approaches have been successfully employed to monitor gene loci dynamics and DNA breaks/repairs in live cells.

Conclusions:

  • Fluorogenic CRISPR strategies represent a significant improvement over conventional tools for live cell DNA imaging.
  • These advancements hold promise for expanding applications in cellular DNA imaging and various bio-applications.