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Updated: Jan 13, 2026

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
Published on: May 10, 2013
Christiangramia qingdaonensis sp. nov., a novel polysaccharide-degrading Bacteroidota bacterium, isolated from
Zhao-Tong Chen1,2, Bing Yu1,2, Yu-Shan Li1,2
1College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, People's Republic of China.
Abstract:
A novel polysaccharide-degrading bacterial strain, designated ASW11-125T, was isolated from intertidal sediments in Aoshan Bay, Qingdao, China. The strain was strictly aerobic, Gram-stain-negative, catalase-positive but oxidase-negative, short rod-shaped, and exhibited gliding motility without flagella. Growth occurred at 4-35°C (optimum 28°C), pH 6.0-8.0 (optimum pH 7.0), and in 0.5-16.0% NaCl (optimum 2.5-3.0%). The predominant polar lipid was phosphatidylethanolamine. Major fatty acids were iso-C15:0 and iso-C17:0 3-OH, and the primary respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic analyses of 16S rRNA gene sequences and 1542 single copy orthologous clusters, strain ASW11-125T affiliated with the genus Christiangramia and was closely related to Christiangramia portivictoriae MCCC 1A00585T (98.8%), Christiangramia aquimixticola KCTC 42706T (98.8%) and Christiangramia marina KCTC 12366T (98.6%). Comparative genomic analysis revealed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain ASW11-125T and its closely related species were 74.6-91.5% and 18.6-44.3%, respectively, which were below the cutoff values for proposing a novel species. Based on a polyphasic characterization integrating phenotypic, phylogenetic, and chemotaxonomic evidence, strain ASW11-125T represents a novel species of the genus Christiangramia, for which the name Christiangramia qingdaonensis sp. nov. is proposed. The draft genome of strain ASW11-125T is 3.2 Mb in size with a G + C content of 38.3%. Notably, genomic analysis revealed an abundance of genes encoding putative carbohydrate-active enzymes (CAZymes), particularly those associated with starch, laminarin, and fructan utilization, suggesting its potential role in the marine carbon cycle. The type strain is ASW11-125T (= KCTC 102340T = MCCC 1K09555T).
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