INVESTIGATING THE EFFECT OF MESENCHYMAL STEM CELLS ON THE PROLIFERATION PHASE OF DUODENAL WOUND HEALING

Area of Science:

• Gastrointestinal Surgery
• Regenerative Medicine
• Wound Healing Research

Background:

• Duodenal perforation is a severe surgical emergency with high mortality.
• Mesenchymal stem cells (MSCs) show promise in wound healing via growth factors and anti-inflammatory cytokines.

Purpose of the Study:

• To evaluate the impact of MSCs on duodenal perforation wound healing.
• To analyze MSCs' effect on key healing markers.

Main Methods:

• Rat model of duodenal perforation treated with two doses of MSCs.
• Control group received saline injection.
• Assessed TGF-β, α-SMA, collagen, and fibroblasts via Western Blot and histopathology on days 3 and 7.

Main Results:

• Increased expression of TGF-β, fibroblasts, collagen, and α-SMA in MSC-treated groups compared to controls.
• Significant enhancement observed on both day 3 and day 7 post-treatment.

Conclusions:

• MSC administration significantly enhances the proliferation phase of duodenal wound healing.
• Increased expression of fibroblasts, collagen, TGF-β, and α-SMA supports MSCs' therapeutic potential.