Related Experiment Video
Updated: May 31, 2026
Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation
Published on: April 5, 2024
Simple Custom Led Panel increases Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells through
Francisca Ancelia1, Isabella Kurnia Liem2,3, Sondang Pandjaitan Sirait4
1Master's Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Introduction:
Impaired proliferation and differentiation capacity of adipose-derived mesenchymal stem cells (AD-MSCs) in obesity hinder the formation of metabolically healthy adipocytes, thereby aggravating metabolic disorders. Photobiomodulation (PBM) is a simple, non-invasive light-based therapy that primarily uses red and near-infrared light and has been shown to enhance the proliferation and differentiation potential of stem cells, including AD-MSCs. However, the wide variation in PBM parameters used in previous studies necessitates further investigation to determine optimal dosage and delivery methods. This study aimed to construct a simple, easy-to-assemble LED panel for PBM application and to identify an effective dose that stimulates proliferation and adipogenic differentiation of AD-MSCs.
Methods:
The panel delivered 1 J/cm2 of 660 nm red light, either daily (P1Pro and P1Diff group) or every other day (P2Pro and P2Diff group).
Results:
No significant differences in total cell count or viability were observed among the groups, indicating that the PBM protocol is safe. KI-67 expression was similar between the P1Pro and control groups (Control: 1.00 ± 0.02; P1Pro: 1.19 ± 0.06, p > 0.05), whereas the P2Pro group showed a significant decrease compared with P1Pro (P2Pro: 0.71 ± 0.06, p = 0.02). The P1Diff group also exhibited a significant increase in expression of adipogenic markers PPARꙋ and CEBPα (1.55 ± 0.04, p < 0.01 and 2.9 ± 0.3, p < 0.01, respectively), while the P2Diff group showed minimal to no difference compared with the control.
Conclusion:
Although further studies are required to assess PBM effects on dysfunctional AD-MSCs from obese individuals, this study demonstrates that a simple, easy-to-assemble PBM device can safely and effectively enhance the proliferation and differentiation capacity of AD-MSCs.
