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Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography
Infection and Immunity
|January 1, 1978
Abstract:
Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previously reported multiple-step procedures.
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