Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exhaled Breath Condensate Reveals PM<sub>2.5</sub>-Driven Airway Metabolic Signature in Young Adults: A Panel Study.

Environmental science & technology·2026
Same author

High-Throughput Single-Cell-Resolved Spatial Proteomics Enabled by an Ordered Colloidal Crystal Column.

Angewandte Chemie (International ed. in English)·2026
Same author

Orthogonal Ionic Liquid-Based Extraction Strategy Enables Amyloid-Specific Profiling of Aggregate Proteome.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Crosslinker nanocarrier-based intratumoral delivery for protein complex mapping in mitochondria of live tumor-bearing mice.

Chemical science·2026
Same author

Resolving the chemical space: a legacy of recording separation science and innovation in <i>Analyst</i>.

The Analyst·2026
Same author

Nonmodified Strategy Enabled Proteome-Wide Mapping of Catechol Derivatives Interactome.

Analytical chemistry·2026

Related Experiment Video

Updated: Jan 13, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.4K

DSPE-Ti-Chip: A Dual-Functionalized Microfluidic Device for High-Efficiency Isolation of Extracellular Vesicle from

Guoshan Hou1,2, Yan Dong3, Min Zhang1

  • 1State Key Laboratory of Medical Proteomics, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Analytical Chemistry
|January 7, 2026
PubMed
Summary

A new microfluidic chip efficiently isolates extracellular vesicles (EVs) from small samples, improving purity and yield compared to traditional methods. This tool aids in understanding lung-on-a-chip models exposed to cigarette smoke and aerosols.

More Related Videos

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.6K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.5K

Related Experiment Videos

Last Updated: Jan 13, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.4K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.6K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.5K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Proteomics

Background:

  • Isolating extracellular vesicles (EVs) from microvolume samples is challenging due to low yield and purity with conventional methods.
  • Existing techniques are often impractical for processing trace biofluid volumes.

Purpose of the Study:

  • To develop a dual-functionalized microfluidic platform for efficient EV isolation from low-volume samples.
  • To compare the performance of the novel platform against ultracentrifugation (UC).
  • To analyze proteomic differences in EVs from lung-on-a-chip models exposed to combustible cigarette smoke (CS) and electronic cigarette aerosols (ECA).

Main Methods:

  • Development of a dual-functionalized microfluidic chip (DSPE-Ti-Chip) utilizing Ti4+ coordination and DSPE phospholipid probes.
  • EV isolation from 20 μL biofluid samples.
  • Comparison of EV yield, purity, and recovery rate with UC.
  • Label-free quantitative proteomics of isolated EVs.

Main Results:

  • The DSPE-Ti-Chip captured twice as many EVs as single-ligand chips.
  • Compared to UC, the chip improved EV purity by 2.2x and yield by 2.5x, with a 5.2x higher recovery rate.
  • Processing time was reduced from 4 hours to 1 hour.
  • Distinct proteomic signatures were identified: CS-EVs showed enrichment in inflammatory proteins, while ECA-EVs were associated with matrix remodeling and cell adhesion.

Conclusions:

  • The DSPE-Ti-Chip is a robust and efficient tool for high-quality EV isolation from limited samples.
  • This platform enables downstream omics analysis.
  • The study reveals divergent pathophysiological pathways induced by CS and ECA exposure via EVs.