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Scalable Multiparametric Characterization of Aptamer-Target Interactions.

Marc Sulliger1, Matthew Peters1, Andrea Sottini1

  • 1Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland.

ACS Nano
|January 8, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a scalable droplet microfluidic platform for analyzing aptamer-target interactions. This biosensing technology enables rapid, detailed characterization of structure-switching aptamers for improved diagnostics.

Keywords:
Förster resonance energy transferbiosensingdroplet microfluidicshyperspectral imagingreaction kineticsserotoninstructure-switching aptamers

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Area of Science:

  • Biotechnology and Biosensing
  • Molecular Biology and Aptamer Engineering

Background:

  • Structure-switching aptamers are crucial for biosensing small molecules by converting conformational changes into signals.
  • Understanding aptamer structural dynamics is key for rational design in biosensor development.
  • Existing methods lack the scalability and spatiotemporal resolution for comprehensive aptamer dynamics analysis.

Purpose of the Study:

  • To develop a scalable droplet microfluidic platform for high-resolution analysis of aptamer-target interactions.
  • To enable multiparametric profiling of aptamer structural dynamics under physiologically relevant conditions.
  • To bridge the gap between structural characterization and biosensor development for data-driven aptamer engineering.

Main Methods:

  • Integration of Förster resonance energy transfer (FRET) with automated imaging in a droplet microfluidic system.
  • Analysis of aptamer-target interactions in picoliter volumes across millisecond-to-hour timescales.
  • Systematic investigation of serotonin aptamers with varying stem lengths to explore structure-function relationships.

Main Results:

  • The platform provides high spatiotemporal resolution for characterizing aptamer structural transitions.
  • Detailed analysis of aptamer-target interactions revealed structure-function relationships.
  • Insights were translated into the selection of optimal aptamer candidates for specific applications.

Conclusions:

  • The developed droplet microfluidic platform overcomes limitations in characterizing aptamer dynamics.
  • This technology facilitates the rational design and engineering of structure-switching aptamers for biosensing.
  • The platform lays the foundation for advancing translational biosensor development and diagnostics.