Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Toxicity Testing in Animals

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dissecting cancer in a non-mammalian model: genomic insights from lemon frost geckos.

BMC biology·2026

Same author

Bias Is Blind to Virtue: Conflicts of Interest across All Sectors of Science.

Environmental science & technology·2026

Same author

A Male Expressed Copulatory Plug Increases Female Fertility in <i>Caenorhabditis</i> Nematodes.

bioRxiv : the preprint server for biology·2026

Same author

Natural Variation in C. elegans 1-Octanol Olfactory Avoidance Behavior.

G3 (Bethesda, Md.)·2026

Same author

Searching for vertically transmitted endosymbionts in over 4,000 wild strains of three self-fertilizing <i>Caenorhabditis</i> species.

microPublication biology·2026

Same author

A gap-free, telomere-to-telomere genome assembly for the Caenorhabditis briggsae reference strain AF16.

G3 (Bethesda, Md.)·2026

Search research articles

Related Experiment Video

Updated: May 6, 2026

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

Published on: May 19, 2011

High-Throughput Toxicity Screening with C. elegans: Current Platforms, Key Advantages, and Future Directions.

Timothy A Crombie¹, Tobias Pamminger², Erik C Andersen³

¹Department of Biomedical Engineering and Science, Florida Institute of Technology, Melbourne, Florida 32901, United States.

Environmental Science & Technology

|January 8, 2026

Summary

Nematodes like Caenorhabditis elegans offer advantages for high-throughput screening (HTS) of chemical toxicity. Image-based HTS data for C. elegans show strong concordance with other species, supporting their use in reducing vertebrate testing.

Keywords:

Caenorhabditis elegans One Health high-throughput assays human health risk assessment toxicity testing

More Related Videos

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

Published on: July 1, 2018

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

Published on: March 14, 2019

Related Experiment Videos

Last Updated: May 6, 2026

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

Published on: May 19, 2011

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

Published on: July 1, 2018

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

Published on: March 14, 2019

Area of Science:

Ecotoxicology
Toxicology
Biotechnology

Background:

Nematodes, particularly Caenorhabditis elegans, are valuable models for high-throughput screening (HTS) due to ease of culture, rapid life cycles, and cost-effectiveness.
Existing HTS platforms for C. elegans assess endpoints like behavior, growth, and reproduction, offering diverse screening capabilities.

Purpose of the Study:

To summarize and evaluate existing HTS methodologies for C. elegans in chemical toxicity screening.
To assess the correlation between C. elegans HTS data and toxicity data from other surrogate species and HTS assays.
To provide recommendations for improving nematode-based HTS platforms and their integration into alternative testing strategies.

Main Methods:

Review of current high-throughput screening (HTS) methodologies for C. elegans.
Comparative analysis of toxicological endpoints between C. elegans and other ecotoxicological surrogates (fish, invertebrates, algae).
Evaluation of image-based HTS data for concordance with established toxicity data.

Main Results:

Nematodes present significant advantages for HTS of chemical toxicity.
Image-based HTS data from C. elegans demonstrated strong concordance with established ecotoxicological surrogate data.
The study identified areas for improvement and collaboration to enhance nematode-based HTS.

Conclusions:

C. elegans HTS platforms are effective tools for chemical toxicity screening.
Nematode-based HTS shows promise as a reliable alternative to vertebrate testing.
Further development and investment are recommended to fully integrate nematodes into toxicity testing batteries.