Related Experiment Video
Updated: Jan 13, 2026
10:29
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
6.9K
The transcription factor AtVIP1 promotes sorghum root development by interacting with the SbARF7 promoter
Lingyan Dai1, Siyu Chai2, Jing Gao3
1Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China. dailingyan770416@126.com.
Plant Cell Reports
|January 8, 2026
Summary
The AtVIP1-SbARF7 module enhances sorghum root development by promoting auxin signaling. This discovery offers insights into root regulatory networks and potential crop improvement strategies.
Area of Science:
- Plant Biology
- Molecular Genetics
- Crop Science
Background:
- Plant root development is intricately regulated by the auxin signaling pathway.
- The Arabidopsis thaliana VirE2-interacting Protein 1 (AtVIP1) gene's role in promoting lateral root growth in sorghum is known, but its precise mechanism remains unclear.
Purpose of the Study:
- To elucidate the molecular mechanism by which AtVIP1 regulates sorghum root development via the auxin signaling pathway.
- To investigate the interaction between AtVIP1 and downstream targets in root growth regulation.
Main Methods:
- Construction and analysis of AtVIP1 overexpressing and silencing lines in sorghum.
- Gene expression analysis, subcellular localization, and hormone treatment experiments.
- Yeast one-hybrid (Y1H), Electrophoretic Mobility Shift Assay (EMSA), and Dual-Luciferase Reporter assays to confirm direct binding and promoter activity.
Main Results:
- AtVIP1 overexpression significantly increased lateral root primordia and root volume, while silencing suppressed root growth.
- AtVIP1 localized to the nucleoplasm and promoted auxin accumulation via the IAA pathway, up-regulating SbYUCCA2 and SbIAA14.
- AtVIP1 directly binds to the SbARF7 promoter, increasing its activity, and this interaction is crucial for promoting root branching.
Conclusions:
- The AtVIP1-SbARF7 module is a key regulator of sorghum root development through auxin signaling.
- This study reveals a novel transcriptional regulatory network for root development.
- Findings provide potential targets for genetic improvement of crop root traits.
Related Concept Videos
01:17Gene Regulation During Sporulation
436
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
436
02:16Transcription Factors
82.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.2K
01:30General Transcription Factors
6.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.7K
01:47Transcription Initiation
20.2K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
20.2K
02:42Eukaryotic Transcription Activators
12.5K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.5K
02:35Transcription Elongation Factors
13.3K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.3K