Engineering Metallic Implants with Self-Catalytic Degradable Bionanozyme Coating and Ion-Releasing Biointerface for...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Implant-associated infections and poor osseointegration are critical issues for titanium (Ti)-based indwelling devices.
• Current strategies often fail to address both infection and bone healing effectively and simultaneously.

Purpose of the Study:

• To develop a multifunctional implant coating with staged antibacterial and osteogenic capabilities.
• To integrate photothermal-enhanced nanobioenzymatic catalysis with degradable release of bioactive components.

Main Methods:

• Fabrication of a Ca²⁺/Cu²⁺-doped titanate layer coated with an alendronate-modified hyaluronic acid (AHA) matrix loaded with a bionanozyme (HMG).
• HMG comprises glucose oxidase (GOx) in hollow MnO₂ nanozymes stabilized by tannic acid (TA).
• Utilized photothermal activity of MnO₂ and TA-mediated Fenton-like reactions for enhanced reactive oxygen species (ROS) generation upon infection.

Main Results:

• Demonstrated bacteria-triggered degradation of the AHA matrix, enabling interface self-renewal and release of HMG for antibacterial action.
• Exposed titanate underlayer promoted cell adhesion and sustained release of Cu²⁺/Ca²⁺ for osteogenesis.
• Confirmed multifaceted antibacterial efficacy, accelerated mineralization, improved tissue healing, and favorable biocompatibility in vitro, ovo, and in vivo studies.

Conclusions:

• The developed nanobioenzymatic coating offers a promising strategy for catalytically active implants with self-adaptive interfaces.
• This approach effectively addresses evolving anti-infective and tissue-regenerative demands in biomedical implants.
• The coating shows potential for enhancing implant integration and patient outcomes by managing infection and promoting bone healing.