Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

1.8K
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
1.8K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

559
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
559
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.6K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.6K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.1K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.1K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

1.6K
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
1.6K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

995
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
995

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zinc modulates metallothionein remodeling and lipid metabolism in human epithelial cells under inflammatory stress.

The Journal of nutritional biochemistry·2026
Same author

Rapid screening of commercial CBD oils by heat-assisted dielectric barrier discharge ionization (HA-DBDI) mass spectrometry and correlation-based fingerprinting.

The Analyst·2026
Same author

Enhancing Biocompatibility: 3D-Printed Cyclic Olefin Copolymer Structures for Advanced Laboratory Applications.

3D printing and additive manufacturing·2025
Same author

Assessment of exposure to pesticides, anticoagulant rodenticides, and PFASs in the blood of top-predator birds by LC-MS/MS analysis.

Environmental research·2025
Same author

Efficient and Wide Chemical-Space Ionization of Organic Contaminants Using LC-MS with a Miniaturized Plasma Source Applying Different Discharge Gases.

Analytical chemistry·2025
Same author

Insight into Desorption Mechanisms in a Helium Low-Temperature Plasma Ionization Source Using Computational Simulations.

Journal of the American Society for Mass Spectrometry·2025
Same journal

AutoPELSA: An Automated Sample Preparation System for Proteome-Wide Identification of Target Proteins of Diverse Ligands.

Analytical chemistry·2026
Same journal

Keeping Reviews Meaningful in the Era of AI.

Analytical chemistry·2026
Same journal

Assessment of Papillary Thyroid Carcinoma by Profiling Multiple Matrix Metalloproteinase Activities Using a Machine Learning-Assisted Peptide Microarray Sensing Platform.

Analytical chemistry·2026
Same journal

Dual-Functional Ratio Photoelectrochemical Biosensing Platform for Photothermal Simultaneous Sterilization and Lipopolysaccharides-Mediated Detection of <i>Escherichia coli</i> O157:H7.

Analytical chemistry·2026
Same journal

Composite Peak Scoring for Improved Charged Aerosol Detector-Based Universal Quantification of Drug Libraries.

Analytical chemistry·2026
Same journal

UV-Triggered Chemiluminescence as a Bulk Surrogate Indicator for Microplastic Contamination in Aquatic Matrices.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 13, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K

An Absolute Quantitative Approach to Study the Desorption Step in Plasma-Based Ambient MS Methods.

Odhisea Gazeli1,2,3, David Moreno-González1, Marcos Bouza1

  • 1Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, Universidad de Jaén, Jaén 23071, Spain.

Analytical Chemistry
|January 9, 2026
PubMed
Summary
This summary is machine-generated.

This study quantifies analyte desorption in plasma-based ambient mass spectrometry (MS). Researchers developed a protocol to precisely measure desorption efficiency, revealing subtle changes with varying conditions.

More Related Videos

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

9.6K
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.6K

Related Experiment Videos

Last Updated: Jan 13, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

9.6K
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.6K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Plasma Science

Background:

  • Ambient mass spectrometry (MS) methods enable sample analysis without extensive preparation.
  • The desorption step is critical for transferring analytes from condensed phases to the gas phase for MS analysis.
  • Thermally assisted desorption is the presumed mechanism in plasma-based ambient MS, but direct evidence is lacking.

Purpose of the Study:

  • To develop and assess a protocol for the absolute quantification of the desorption step in plasma-based ambient MS.
  • To evaluate the desorption efficiency of low-temperature plasma (LTP) using model analytes.
  • To investigate the influence of different LTP conditions on analyte desorption.

Main Methods:

  • A quantitative protocol was established using liquid chromatography/tandem mass spectrometry (LC-MS/MS).
  • Model analytes (arginine, cocaine, rhodamine G, imazalil) were deposited on glass substrates and exposed to varying LTP conditions.
  • Spatially resolved fluorescence microscopy was used to assess analyte distribution on the substrate.

Main Results:

  • The developed protocol allows precise quantification of desorbed analytes with high reproducibility (RSD ≤ 7%).
  • Subtle changes in desorption efficiency (picomole range) were detected by altering discharge gas and exposure time.
  • Analyte deposition was found to be uneven, but the quantification method proved robust to sample deposition variations.

Conclusions:

  • A reliable method for absolute quantification of the desorption step in plasma-based ambient MS was successfully demonstrated.
  • The study provides quantitative data on desorption efficiency under varying LTP conditions, offering insights into the desorption mechanism.
  • Factors such as analyte-analyte interactions and sample thickness may play a role in plasma-based desorption.