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Updated: Jan 9, 2026

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Enhancing Biocompatibility: 3D-Printed Cyclic Olefin Copolymer Structures for Advanced Laboratory Applications.

Simon Höving1, Stefanie Dörr1, Marc Akermann2

  • 1Translationale Forschung, Miniaturisierung, Leibniz-Institut für Analytische Wissenschaften - ISAS e.V., Dortmund, Germany.

3D Printing and Additive Manufacturing
|December 4, 2025
PubMed
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This summary is machine-generated.

3D-printed cyclic olefin copolymer (COC) and Glass + COC show promising biocompatibility for cell-based research, with comparable or improved cell viability versus standard polystyrene. Material selection is key for optimizing cell culture applications.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Additive Manufacturing

Background:

  • Additive manufacturing, or 3D-printing, is vital for creating prototypes and specialized components in scientific fields.
  • Biocompatibility of 3D-printed materials is critical for cell-based research and millifluidic applications, influencing cell culture and interactions with reactive substances.

Purpose of the Study:

  • To investigate the biocompatibility and performance of 3D-printed cyclic olefin copolymer (COC) and polylactic acid (PLA) compared to traditional materials.
  • To assess the impact of these materials on cell viability, metabolic activity, and potential toxicity using specific cell lines.

Main Methods:

  • Experiments utilized rat cardiomyocyte (H9c2) and human embryonal kidney (HEK293) cell lines.
  • Assays included lactate, lactate dehydrogenase (LDH), and thiazolyl blue tetrazolium bromide to evaluate metabolic activity, cell stress, and viability.
Keywords:
additive manufacturingbiocompatibilitycell viabilitycyclic olefin copolymer (COC)extrusion 3D-printinglaboratory applications

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  • Microscopy and atomic force microscopy (AFM) were employed to visualize cell growth and analyze surface characteristics.

    • Main Results:

    • Glass + COC demonstrated increased metabolic activity and cell viability compared to standard polystyrene (PS) dishes.
    • COC and PLA materials showed comparable cell viability to standard PS dishes, with a slight advantage for COC.
    • Lactate assays indicated subtle increases in secretion, particularly with Glass + COC, correlating with cell viability.

    Conclusions:

    • 3D-printed COC and Glass + COC are biocompatible materials suitable for cell-based research and millifluidic applications.
    • Material selection significantly impacts cell viability, metabolic activity, and lactate levels in cell culture.
    • Microscopy and AFM analyses provide crucial insights into cell behavior and material properties for optimizing biocompatible 3D-printed applications.