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Updated: Jan 13, 2026
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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
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Development of a DMSNs@CuInS/ZnS-based flow immunochromatographic strip method for gliadin detection
Lingjie Sheng1, Xiangyang Liu1, Yafei Zhang1
1State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
Talanta
|January 9, 2026
Summary
A new fluorescent lateral flow immunoassay (LFIA) uses quantum dots in nanoparticles for sensitive gliadin detection. This method offers accurate gluten monitoring for managing food allergies.
Area of Science:
- Analytical Chemistry
- Materials Science
- Biotechnology
Background:
- Gliadin detection is crucial for managing celiac disease and gluten sensitivity.
- Existing methods for gliadin detection may lack sensitivity or speed.
- Development of rapid, sensitive, and accurate diagnostic tools is needed.
Purpose of the Study:
- To develop a highly sensitive fluorescent lateral flow immunoassay (LFIA) for gliadin detection.
- To utilize CuInS/ZnS quantum dots loaded onto dendritic mesoporous silica nanoparticles (DMSNs) as signal reporters.
- To enhance signal amplification for improved detection limits.
Main Methods:
- Fabrication of DMSNs loaded with CuInS/ZnS quantum dots.
- Development of a fluorescent LFIA assay for gliadin detection.
- Optimization of assay conditions and validation with real food samples.
Main Results:
- Achieved a wide linear range (12.50 ± 0.47 μg/L to 1200.00 ± 1.69 μg/L).
- Obtained a very low limit of detection (LOD) of 0.02 ± 0.01 μg/L.
- Demonstrated high spiked recovery rates (82.19%-116.57%) in various food matrices (biscuit, bread, flour).
Conclusions:
- The developed LFIA provides a rapid, sensitive, and accurate method for gliadin detection.
- The use of DMSNs loaded with quantum dots enhances signal amplification.
- This assay is a reliable tool for gluten monitoring and managing gluten-related allergies.
