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Organoid chip based automatic system for long-term quantifying ciliary beating under drug intervention.

Zixi Li1, Zhicheng Huang2, Daoyun Wang2

  • 1Department of Biomedical Engineering, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.

Analytica Chimica Acta
|January 9, 2026
PubMed
Summary
This summary is machine-generated.

A new microfluidic chip system (AuCilia) enables precise, long-term monitoring of human-derived ciliary organoids (HDCOs). This technology overcomes limitations in studying ciliary beating and accelerates drug discovery for related diseases.

Keywords:
Ciliary beatingDrug evaluationHuman-derived organoidMicrofluidic chip

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Pharmacology

Background:

  • Motile cilia are crucial for physiological processes, and their dysfunction is linked to various diseases.
  • Current in vitro models lack the complexity of in vivo systems, hindering research.
  • Human-derived ciliary organoids (HDCOs) offer a promising bridge but face challenges with limited numbers and tracking accuracy.

Purpose of the Study:

  • To develop a system for precise, long-term monitoring and analysis of ciliary beating in HDCOs.
  • To overcome technical barriers in HDCO culture and observation, such as limited sample size and organoid displacement.
  • To create a platform for efficient drug screening and physiological studies of cilia.

Main Methods:

  • Development of a multifunctional microfluidic chip (MOCiB-Chip) with integrated immobilization, culture, observation, and drug mixing capabilities.
  • Establishment of an automated quantifying system for ciliary beating (AuCilia) using the MOCiB-Chip, Ciliary Beating Frequency Analysis Software (CBFAS), and a fluid control module.
  • 7-day monitoring of human bronchial HDCOs and evaluation of roflumilast's effects on ciliary beating frequency and cAMP levels.

Main Results:

  • The AuCilia system successfully achieved 7-day monitoring of HDCOs, maintaining tracking accuracy.
  • Roflumilast demonstrated dose-dependent effects: 10 nM increased beating frequency by 81.5%, 1 nM by 27.8%, while 100 nM caused cessation by day 2.
  • cAMP measurements validated the mechanism of action for roflumilast, confirming the system's reliability.

Conclusions:

  • The developed strategy integrates all procedures on-chip, eliminating HDCO loss and resolving conflicts between limited samples and multi-condition testing.
  • Improved tracking accuracy by preventing organoid displacement/overlapping overcomes multi-well-plate limitations.
  • The low-cost, reproducible system accelerates drug screening and enables precise study of cilia physiology, supporting research and clinical translation for cilia-related diseases.