Related Experiment Video
Updated: Jan 13, 2026
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
Published on: April 30, 2014
21.1K
Molecular Characterization and Functional Insights into Goose IGF2BP2 During Skeletal Muscle Development
Cui Wang1, Yi Liu1, Jiuli Dai2
1Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Animals : an Open Access Journal From MDPI
|January 10, 2026
Summary
We characterized goose Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), finding high expression in muscle tissues. Overexpression in cells suggests IGF2BP2 plays a key role in goose skeletal muscle development.
Area of Science:
- Genomics and Molecular Biology
- Animal Science
- Developmental Biology
Background:
- Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is crucial for metabolism, cell proliferation, and tumorigenesis.
- While its role in muscle development is known in other species, goose IGF2BP2 function is understudied.
Purpose of the Study:
- To clone and characterize the full-length cDNA and genomic DNA of goose IGF2BP2.
- To investigate the expression patterns of goose IGF2BP2 in different tissues and developmental stages.
- To explore the functional role of IGF2BP2 in goose skeletal muscle development.
Main Methods:
- Full-length cDNA and genomic DNA sequencing of goose IGF2BP2.
- Identification of genetic variants, including frameshift mutations.
- Quantitative analysis of IGF2BP2 mRNA expression in various goose tissues and developmental stages.
- Overexpression of IGF2BP2 in goose skeletal muscle satellite cells (SMSCs) followed by gene expression analysis and protein-protein interaction network analysis.
Main Results:
- The goose IGF2BP2 cDNA is 2957 bp, encoding a 553-amino acid protein with five RNA-binding domains. The genomic sequence spans 12,183 bp with 12 exons and 11 introns.
- Sixty genetic variants were identified, including a frameshift mutation (g.2299delG).
- High IGF2BP2 mRNA levels were observed in liver, heart, and muscle tissues of female geese at embryonic, growing, and laying stages (p < 0.05).
- Overexpression of IGF2BP2 in SMSCs significantly altered the expression of genes involved in muscle development and signaling pathways, including upregulation of IGF1, EGFR, FGF19, BMP6, BMP2, ACVR1C, and WNT5A, and downregulation of MYBPC3, NODAL, HOXD13, TNXB, and ADD2 (Padj < 0.01).
Conclusions:
- Goose IGF2BP2 possesses conserved RNA-binding domains and exhibits significant genetic variation.
- High expression in key tissues suggests a role in goose development, particularly muscle development.
- IGF2BP2 may regulate skeletal muscle development in geese by coordinating the expression of critical genes within signaling pathways.
Related Concept Videos
01:13Formation of Muscle Fibers from Myoblasts
5.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.8K
01:30General Transcription Factors
6.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.7K
02:23Master Transcription Regulators
7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
02:21Globular and Fibrous Proteins
46.7K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
46.7K