Overexpression of GmCSY3 Enhances Soybean Tolerance to Excess Iron and Aluminum
Zhuo Liu1, Hongqiu Lv1, Liying Yang1
1Heilongjiang Provincial Key Laboratory of Molecular Cell Genetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin 150080, China.
Background:
Plant citrate synthase (CSY) is involved in the iron deficiency (-Fe) response and aluminum (Al) detoxification. However, knowledge of CSY function in responding to excess iron (+Fe) or Al stress (+Al) is still limited.
Methods:
The CDS and promoter of GmCSY3 were isolated from soybean and bioinformatically analyzed. The GmCSY3 expression was detected by qRT-PCR and GUS assay. The growth of GmCSY3 recombinant yeast under +Fe or +Al was detected. The phenotype, CSY activity, citric acid concentration, chlorophyll content, MDA, H2O2, O2- contents, GST, CAT, SOD, and POD activities were examined in GmCSY3 overexpressed and RNAi-suppressed soybean chimeras under +Fe or +Al. Perls and Hematoxylin stained the roots, and the FCR activity was determined.
Results:
GmCSY3 was induced by +Fe or +Al, but not by -Fe. GmCSY3 enhanced yeast's acid production and resistance to +Fe or +Al. GmCSY3 overexpression in soybean significantly enhanced CSY activity, promoted growth, alleviated oxidative damage caused by +Fe or +Al, with less free Fe3+ and Al3+, and reduced FCR activity, while GmCSY3 RNAi-suppressed showed the opposite effect.
Conclusions:
GmCSY3 promotes the process of citrate synthesis, chelates Fe3+ and Al3+, alleviates oxidative damage caused by +Fe or +Al, and modulates iron absorption in plants.
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