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Updated: Jan 13, 2026
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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
25.8K
TurboID-Based Proximity Labeling in Plants
Chaonan Shi1, Huang Tan2, Rosa Lozano-Durán3
1Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen, Germany. chaonan.shi@uni-tuebingen.de.
Methods in Molecular Biology (Clifton, N.J.)
|January 10, 2026
Summary
This chapter details a TurboID proximity labeling protocol for identifying plant protein interactions. It guides researchers from creating fusion proteins to analyzing results using the Ti-TAN plasmid collection.
Area of Science:
- Biochemistry
- Molecular Biology
- Plant Science
Background:
- Proximity labeling (PL) techniques are vital for studying protein-protein interactions in living organisms.
- Understanding protein associations is crucial for deciphering cellular processes in plants.
Purpose of the Study:
- To present a detailed, step-by-step protocol for TurboID-based proximity labeling in plants.
- To enable the comprehensive definition of a protein's "proxiome" (interacting partners) in a plant context.
Main Methods:
- Utilizing the TurboID enzyme for proximity-dependent biotinylation.
- Employing the publicly available Ti-TAN plasmid collection for generating fusion proteins.
- A comprehensive workflow from fusion protein generation to data analysis.
Main Results:
- The protocol facilitates the identification of proximal proteins to a protein of interest.
- Successful application of TurboID-based PL for mapping plant protein interactomes.
- The Ti-TAN plasmid collection simplifies the experimental setup.
Conclusions:
- This TurboID-based proximity labeling protocol provides a robust method for plant interactome studies.
- The presented workflow empowers researchers to investigate protein associations in vivo.
- This approach enhances the understanding of plant molecular mechanisms.
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