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Updated: Jan 13, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Chromosome-level genome assembly of the dwarf cattail Typha minima
Junshuai Du1, Lei Huang1, Xinwei Xu2
1National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Sciences, Wuhan University, Wuhan, P. R. China.
Abstract:
We report the first chromosome-level genome assembly of the critically endangered dwarf cattail, Typha minima, a wetland species of ecological and medicinal importance. Utilizing PacBio HiFi long-read sequencing and Hi-C scaffolding technologies, we generated a high-quality 324.66 Mb genome, anchored onto 30 pseudochromosomes. The assembly demonstrates exceptional continuity, with contig and scaffold N50 values of 10.84 Mb and 10.90 Mb, respectively, and a near-complete chromosomal anchoring rate of 99.65%. It exhibits outstanding completeness, as reflected by a BUSCO score of 99.2%, and contains 33.20% repetitive sequences. We annotated 34,541 protein-coding genes, with 96.42% receiving functional assignments. The assembly also includes annotations for non-coding RNAs, comprising 1,261 rRNAs, 230 miRNAs, and 467 tRNAs. Integrated orthology analysis identified 10,055 consensus orthologs across five functional databases. This high-quality genomic resource provides a foundation for advancing studies in evolutionary adaptation and conservation genomics of this endangered wetland plant.
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