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Advances in Optical Biosensors for Pesticide Detection.
Meini Zhao1,2,3, Yijie Wang2, Rui Jin1,2
1Department of Food Quality and Safety College of Food Science and Engineering, Jilin University, Changchun 130062, P. R. China.
Research (Washington, D.C.)
|January 12, 2026
Summary
Optical biosensors offer sensitive and specific detection of pesticide residues for food safety and environmental monitoring. This review summarizes advancements in fluorescence, colorimetry, SERS, SPR, and chemiluminescence strategies for pesticide analysis.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Environmental Science
Background:
- Growing concerns over pesticide residues in food and the environment necessitate advanced detection methods.
- Optical biosensors provide a promising platform for sensitive and specific pesticide detection.
Purpose of the Study:
- To comprehensively review fundamental knowledge and recent advancements in optical biosensors for pesticide detection.
- To highlight the advantages of optical biosensors, including sensitivity, specificity, and on-site applicability.
Main Methods:
- Review of various optical biosensing techniques: fluorescence (FL), colorimetry (CL), surface-enhanced Raman scattering (SERS), surface plasmon resonance (SPR), and chemiluminescence.
- Detailed overview of sensing mechanisms based on molecular recognition elements like enzymes, antibodies, aptamers, molecularly imprinted polymers (MIPs), and host-guest complexes.
Main Results:
- Optical biosensors demonstrate excellent sensitivity, high specificity, and potential for on-site pesticide detection.
- Diverse molecular recognition elements enable tailored sensing strategies for various pesticides.
Conclusions:
- Optical biosensors are crucial tools for ensuring food safety and environmental integrity by detecting pesticide residues.
- Future research should focus on overcoming current challenges to further enhance the performance and expand the applications of pesticide biosensors.

