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Published on: January 10, 2015
Release of Pro-Inflammatory Cytokines from Macrophages Stimulated by Denture Base Materials in Smokeless Tobacco
Karabi Das1, Mohit Singh Raghuvanshi2, Apurv Mehrotra3
1Department of Oral Medicine and Radiology, Regional Dental College, Guwahati, Assam, India.
Background:
Denture base materials, when exposed to carcinogenic substances like smokeless tobacco extracts (STE), may provoke inflammatory responses in oral tissues. Understanding macrophage-mediated cytokine release in such environments is critical to evaluating the biocompatibility of these materials.
Materials And Methods:
Three denture base materials-conventional heat-cured poly (methyl methacrylate) (PMMA), high-impact PMMA, and CAD/CAM-milled PMMA-were evaluated. RAW 264.7 macrophages were exposed to material eluates with and without STE. Cytokine levels were quantified using ELISA.
Results:
All materials showed significantly increased cytokine release in STE environments, with conventional PMMA eliciting the highest levels. CAD/CAM PMMA showed the least cytokine induction.
Conclusion:
Exposure to smokeless tobacco enhances the pro-inflammatory potential of denture base materials, with conventional PMMA being the most reactive. CAD/CAM PMMA demonstrates superior biocompatibility in inflammatory conditions.
