Fluoro-forest: a random forest workflow for cell type annotation in high-dimensional immunofluorescence imaging with...

Cyclic immunofluorescence (IF) techniques enable deep phenotyping of cells and help quantify tissue organization at high resolution. Due to its high dimensionality, workflows typically rely on unsupervised clustering, followed by cell type annotation at a cluster level for cell type assignment. Most of these methods use marker expression averages that lack a statistical evaluation of cell type annotations, which can result in misclassification. Here, we propose a strategy through an end-to-end pipeline using a semi-supervised, random forest approach to predict cell type annotations.

Our method includes cluster-based sampling for training data, cell type prediction, and downstream visualization for interpretability of cell annotation that ultimately improves classification results. We show that our workflow can annotate cells more accurately compared to representative deep learning and probabilistic methods, with a training set <5% of the total number of cells tested. In addition, our pipeline outputs cell type probabilities and model performance metrics for users to decide if it could boost their existing clustering-based workflow results for complex IF data.

Fluoro-forest is freely available on GitHub under an MIT license (https://github.com/Josh-Brand/Fluoro-forest).