Related Experiment Video
Updated: Jan 13, 2026

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
Published on: May 5, 2021
Transient High-Glucose Exposure and Erk Signaling
Junichi Okada1, Tsugumichi Saito2, Eijiro Yamada2
1Department of Medicine, Division of Endocrinology, Albert Einstein College of Medicine Bronx, NY USA.
Background:
Transient high-glucose exposure induces Erk2 phosphorylation and cell proliferation in human coronary artery endothelial cells (HCAEC), but the upstream mechanisms remain unclear.
Methods And Results:
HCAEC were exposed to 183 mg/dL L-glucose or D-glucose for 1 h followed by 23 h in a glucose-free medium. Transient high D-glucose decreased miR-138 levels, increased Giα2 protein levels, inactivated Rap1, and activated Ras, leading to Erk2 activation.
Conclusions:
Transient high D-glucose exposure activates Erk2 via the miRNA-138/Giα2/Rap1/Ras signaling axis, suggesting a potential therapeutic target for atherosclerosis.
More Related Videos
Related Concept Videos
cAMP-dependent Protein Kinase Pathways
Cell Specific Gene Expression
Insulin: The Receptor and Signaling Pathways
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Hypoglycemia and Glucagon
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...

