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Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model
Published on: August 4, 2017
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Thermosensitive Hydrogel Derived from a Human Amniotic Membrane Promotes Diabetic Wound Healing
Pratibha Jaipal1, Sunil Gujjar1, Shubhanshi Ranjan1
1BRIC-Translational Health Science and Technology Institute, Faridabad 121001, Haryana, India.
ACS Biomaterials Science & Engineering
|January 15, 2026
Summary
Human amniotic membrane (AM) extracellular matrix (ECM) hydrogels promote diabetic wound healing. These AM ECM hydrogels accelerate closure, reduce inflammation, and decrease fibrosis in diabetic mice.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Extracellular matrix (ECM) hydrogels are crucial for regenerative medicine, mimicking native tissue microenvironments.
- Human amniotic membrane (AM) is a rich source of ECM with inherent wound-healing properties and minimal ethical concerns.
Purpose of the Study:
- To develop and characterize thermosensitive hydrogels from decellularized AM (AM ECM hydrogels).
- To evaluate the therapeutic potential of AM ECM hydrogels for diabetic wound healing.
Main Methods:
- Detergent-enzymatic decellularization of AM to obtain ECM.
- Lyophilization, cryomilling, and pepsin digestion to prepare hydrogel precursors.
- Physicochemical characterization (gelation, swelling, porosity, mechanics, degradation).
- In vitro biological evaluation using skin cells and endothelial cells.
- In vivo assessment in a diabetic murine wound model.
Main Results:
- AM ECM hydrogels exhibited temperature-dependent gelation, high water content (>97%), and >60% porosity.
- In vitro studies showed high cell viability (>70%), significant proliferation (>300%), preserved cytoskeleton, and minimal apoptosis.
- Proteomic analysis confirmed retention of key matrisome proteins involved in repair.
- In vivo studies demonstrated accelerated wound closure, vascular stabilization, and reduced inflammation and fibrosis in diabetic mice.
Conclusions:
- Decellularized AM can be processed into thermosensitive ECM hydrogels with favorable physicochemical properties.
- AM ECM hydrogels support cell viability and function, promoting key aspects of tissue repair.
- AM ECM hydrogels effectively accelerate diabetic wound healing by modulating the inflammatory and fibrotic responses.
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