Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multicenter study on the prediction model for chronic low back pain after lumbar decompression surgery in patients with diabetes mellitus: integration of metabolic and paraspinal muscle features.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Corrigendum to "Bone marrow mesenchymal stem cells senescence induced by LCCP through activation of cGAS-STING-mediated inflammation" [Ecotoxicol. Environ. Saf. 294 (2025) 118069].

Ecotoxicology and environmental safety·2026
Same author

Transport of Engineered Nanoparticles in Solid Tumors: Mechanistic Barriers and Emerging Delivery Strategies.

Bioconjugate chemistry·2026
Same author

Association of glucose metabolism status and paraspinal muscle degeneration with chronic pain after endoscopic lumbar decompression: a multicentre retrospective study.

Frontiers in endocrinology·2026
Same author

Spatiotemporal regulation of neutrophil-mediated immune cascades via engineered PEEK surfaces restores osseointegration in diabetes.

Bioactive materials·2026
Same author

Satellite time-series mapping and driving mechanisms of cropland abandonment in mountainous regions of China.

Journal of environmental management·2026

Related Experiment Video

Updated: Jan 16, 2026

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

5.2K

A Microfibrous Extracellular Matrix Platform for In Vitro Hepatotoxicity Screening: A Next-Generation Model for

Navneet Kaur1,2, Mary Jean Savitsky1,2, Michael Lipscomb3

  • 1Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Tallahassee, Florida 32310, United States.

ACS Pharmacology & Translational Science
|January 15, 2026
PubMed
Summary

A novel 3D culture system using solid microfibrous extracellular matrix (smfECM) improves hepatocyte function and drug-induced liver toxicity assessment. This advanced model enhances drug screening accuracy and shows promise for in vivo applications.

Keywords:
drug-induced liver toxicityextracellular matrixhepatocyteshepatotoxicityliver tissue engineeringspheroids

More Related Videos

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

7.2K
Author Spotlight: Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications
07:23

Author Spotlight: Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications

Published on: October 20, 2023

1.8K

Related Experiment Videos

Last Updated: Jan 16, 2026

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

5.2K
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
08:25

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

7.2K
Author Spotlight: Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications
07:23

Author Spotlight: Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications

Published on: October 20, 2023

1.8K

Area of Science:

  • Biomedical Engineering
  • Hepatology
  • Drug Development

Background:

  • Drug-induced hepatotoxicity poses risks in drug development and patient safety.
  • Traditional 2D cell cultures inadequately model the liver's complex in vivo environment for toxicity testing.
  • Accurate assessment of drug-induced liver injury is crucial for pharmaceutical research.

Purpose of the Study:

  • To develop an advanced 3D cell culture model for improved hepatotoxicity assessment.
  • To evaluate the efficacy of a solid microfibrous extracellular matrix (smfECM) for culturing hepatocytes.
  • To enhance the accuracy of high-throughput drug screening and toxicity evaluation.

Main Methods:

  • Culturing human and mouse hepatocytes in a 3D smfECM scaffold.
  • Assessing hepatocyte growth, viability, and function (albumin secretion, urea production).
  • Confirming metabolic function via CYP3A4 expression and evaluating in vivo performance after implantation.

Main Results:

  • The 3D smfECM system demonstrated superior hepatocyte growth, viability, and functionality compared to 2D cultures.
  • Hepatocytes in 3D spheroids maintained normal metabolic functions, including CYP3A4 expression.
  • Drug screening using smfECM showed high accuracy, aligning with clinical hepatotoxicity data.
  • In vivo implantation confirmed cell survival, angiogenesis promotion, and lack of immunogenicity.

Conclusions:

  • The 3D smfECM offers a reliable, humanized model for accurate hepatotoxicity assessment in drug development.
  • This advanced model improves high-throughput drug screening and toxicity evaluation.
  • smfECM represents a promising tool for safer and more effective pharmaceutical research.